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PPP2R5C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP2R5C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPP2R5C antibodies →
$504.00each
Catalog Number: LS-G128213-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP2R5C (NM_001161726), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
B alpha regulatory subunit | B56G | KIAA0044 | PPP2R5C | PR61G | PP2A B subunit isoform B-gamma
Origin Species
Human
NCBI GenBank
NM_002719
SwissProt
Q13362

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPNKNKKEKE SPKAGKSGKS SKEGQDTVES EQISVRKNSL VAVPSTVSAK IKVPVSQPIV
61KKDKRQNSSR FSASNNRELQ KLPSLKDVPP ADQEKLFIQK LRQCCVLFDF VSDPLSDLKW
121KEVKRAALSE MVEYITHNRN VITEPIYPEV VHMFAVNMFR TLPPSSNPTG AEFDPEEDEP
181TLEAAWPHLQ LVYEFFLRFL ESPDFQPNIA KKYIDQKFVL QLLELFDSED PRERDFLKTT
241LHRIYGKFLG LRAYIRKQIN NIFYRFIYET EHHNGIAELL EILGSIINGF ALPLKEEHKI
301FLLKVLLPLH KVKSLSVYHP QLAYCVVQFL EKDSTLTEPV VMALLKYWPK THSPKEVMFL
361NELEEILDVI EPSEFVKIME PLFRQLAKCV SSPHFQVAER ALYYWNNEYI MSLISDNAAK
421ILPIMFPSLY RNSKTHWNKT IHGLIYNALK LFMEMNQKLF DDCTQQFKAE KLKEKLKMKE
481REEAWVKIEN LAKANPQAQK DPKKDRPLAR RKSELPQDPH TKKALEAHCR ADELASQDGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

540 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.