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PPP2R3C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP2R3C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112121-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP2R3C (NM_017917), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G4-1 | G5pr | PPP2R3C | C14orf10
Origin Species
Human
NCBI GenBank
NM_017917
SwissProt
Q969Q6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MDWKEVLRRR LATPNTCPNK KKSEQELKDE EMDLFTKYYS EWKGGRKNTN EFYKTIPRFY
61YRLPAEDEVL LQKLREESRA VFLQRKSREL LDNEELQNLW FLLDKHQTPP MIGEEAMINY
121ENFLKVGEKA GAKCKQFFTA KVFAKLLHTD SYGRISIMQF FNYVMRKVWL HQTRIGLSLY
181DVAGQGYLRE SDLENYILEL IPTLPQLDGL EKSFYSFYVC TAVRKFFFFL DPLRTGKIKI
241QDILACSFLD DLLELRDEEL SKESQETNWF SAPSALRVYG QYLNLDKDHN GMLSKEELSR
301YGTATMTNVF LDRVFQECLT YDGEMDYKTY LDFVLALENR KEPAALQYIF KLLDIENKGY
361LNVFSLNYFF RAIQELMKIH GQDPVSFQDV KDEIFDMVKP KDPLKISLQD LINSNQGDTV
421TTILIDLNGF WTYENREALV ANDSENSADL DDTTRTRPLE QKLISEEDLA ANDILDYKDD
481DDKV

484 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.