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PPP2R3B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP2R3B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114382-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP2R3B (NM_013239), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NY-REN-8 antigen | NYREN8 | PP2A subunit B isoform PR48 | PPP2R3LY | PP2A | subunit B | PR48 isoform | PPP2R3B | PPP2R3L | PR48
Origin Species
Human
NCBI GenBank
NM_013239
SwissProt
Q9Y5P8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPPGKVLQPV LKMKVDELFL YWLSEASTQR MLQDCLRRIK APGRDQPTPG DGEQPGAWPT
61APLAAPRPSG LEPPGTPGPG PALPLGAASS PRNAPHVRGT RRSAGTRVVQ TRKEEPLPPA
121TSQSIPTFYF PRGRPQDSVN VDAVISKIES TFARFPHERA TMDDMGLVAK ACGCPLYWKG
181PLFYGAGGER TGSVSVHKFV AMWRKILQNC HDDAAKFVHL LMSPGCNYLV QEDFVPFLQD
241VVNTHPGLSF LKEASEFHSR YITTVIQRIF YAVNRSWSGR ITCAELRRSS FLQNVALLEE
301EADINQLTEF FSYEHFYVIY CKFWELDTDH DLLIDADDLA RHNDHALSTK MIDRIFSGAV
361TRGRKVQKEG KISYADFVWF LISEEDKKTP TSIEYWFRCM DLDGDGALSM FELEYFYEEQ
421CRRLDSMAIE ALPFQDCLCQ MLDLVKPRTE GKITLQDLKR CKLXNVFFDT FFNIEKYLDH
481EQKEQISLLR DGDSGGPELS DWEKYAAEEY DILVAEEXXG EPWEDGFEAE LSXVEQKLSA
541LRSPLAQRPF FEAPSPLGAV DLYEYACGDE DLEPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

575 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.