Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPP1R9B / Spinophilin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108708-100
Catalog Number: LS-G108708
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP1R9B(PPP1R9B) (NM_032595), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PPP1R9 | Spinophilin | SPINO | Neurabin-2 | Neurabin II | Neurabin-II | PPP1R6 | PPP1R9B
- Origin Species
- Human
- NCBI GenBank
- NM_032595
- SwissProt
- Q96SB3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 89.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMKTEPRGPG GPLRSASPHR SAYEAGIQAL KPPDAPGPDE APKGAHHKKY GSNVHRIKSM61FLQMGTTAGP SGEAGGGAGL AEAPRASERG VRLSLPRASS LNENVDHSAL LKLGTSVSER121VSRFDSKPAP SAQPAPPPHP PSRLQETRKL FERSAPAAAG GDKEAAARRL LRQERAGLQD181RKLDVVVRFN GSTEALDKLD ADAVSPTVSQ LSAVFEKADS RTGLHRGPGL PRAAGVPQVN241SKLVSKRSRV FQPPPPPPPA PSGDAPAEKE RCPAGQQPPQ HRVAPARPPP KPREVRKIKP301VEVEESGESE AESAPGEVIQ AEVTVHAALE NGSTVATAAS PAPEEPKAQA APEKEAAAVA361PPERGVGNGR APDVAPEEVD ESKKEDFSEA DLVDVSAYSG LGEDSAGSAL EEDDEDDEED421GEPPYEPESG CVEIPGLSEE EDPAPSRKIH FSTAPIQVFS TYSNEDYDRR NEDVDPMAAS481AEYELEKRVE RLELFPVELE KDSEGLGISI IGMGAGADMG LEKLGIFVKT VTEGGAAHRD541GRIQVNDLLV EVDGTSLVGV TQSFAASVLR NTKGRVRFMI GRERPGEQSE VAQLIQQTLE601QERWQREMME QRYAQYGEDD EETGEYATDE DEELSPTFPG GEMAIEVFEL AENEDALSPV661DMEPEKLVHK FKELQIKHAV TEAEIQQLKR KLQSLEQEKG RWRVEKAQLE QSVEENKERM721EKLEGYWGEA QSLCQAVDEH LRETQAQYQA LERKYSKAKR LIKDYQQKEI EFLKKETAQR781RVLEESELAR KEEMDKLLDK ISELEGNLQT LRNSNSTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
817 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.