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PPP1R3G Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP1R3G Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPP1R3G antibodies →
$504.00each
Catalog Number: LS-G126533-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP1R3G (NM_001145115), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PPP1R3G
Origin Species
Human
NCBI GenBank
NM_001145115
SwissProt
B7ZBB8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
37.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPIGARLSL EAPGPAPFRE APPAEELPAP VVPCVQGGGD GGGASETPSP DAQLGDRPLS
61PKEEAAPQEQ EELLECRRRC RARSFSLPAD PILQAAKFLQ QQQQQAVALG GEGAEDAQLG
121PGGCCAKCKK RVQFADTLGL SLASVKHFSE AEEPQVPPAV LSRLRSFPMR AEDLEQLGGL
181LAAAAVAAPL SAPPSRLRPL FQLPGPSAAA ERLQRQRVCL ERVQCSTASG AEVKGSGRVL
241SCPGPRAVTV RYTFTEWRSF LDVPAELQPE PLEPQQPEAP SGASEPGSGD AKKEPGAECF
301HFSLCLPPGL QPEDEEDADE RGVAVHFAVC YRCAQGEYWD NNAGANYTLR YARPADAL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

358 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.