Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPP1R3C / PTG Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115991-100
Catalog Number: LS-G115991
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP1R3C (NM_005398), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PP1 subunit R5 | PTG | PPP1R3C | PPP1R5 | Protein targeting to glycogen
- Origin Species
- Human
- NCBI GenBank
- NM_005398
- SwissProt
- Q9UQK1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSCTRMIQVL DPRPLTSSVM PVDVAMRLCL AHSPPVKSFL GPYDEFQRRH FVNKLKPLKS61CLNIKHKAKS QNDWKCSHNQ AKKRVVFADS KGLSLTAIHV FSDLPEEPAW DLQFDLLDLN121DISSALKHHE EKNLILDFPQ PSTDYLSFRS HFQKNFVCLE NCSLQERTVT GTVKVKNVSF181EKKVQIRITF DSWKNYTDVD CVYMKNVYGG TDSDTFSFAI DLPPVIPTEQ KIEFCISYHA241NGQVFWDNND GQNYRIVHVQ WKPDGVQTQM APQDCAFHQT SPKTELESTI FGSPRLASGL301FPEWQSWGRM ENLASYRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
317 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.