Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPP1R36 / C14orf50 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105462-100
Catalog Number: LS-G105462
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C14orf50(PPP1R36) (NM_172365), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C14orf50 | PPP1R36
- Origin Species
- Human
- SwissProt
- Q96LQ0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MYRVPEFYAR RKRLGGQTPY LMDQLGLRLG MWYWKDETRT LEFRRFAAED SVQWLLKHHP61HFTPAAEVKE KGKKGKAVHF AEIDGPASDR LTDKRLAAKD DKSAKAVEKR GQQGTITLDD121VKFVTLLLLQ DTEMQRICSF TTFMRNKNLD NFLMALLYYL SHYLEKNSLE KKPKSYMVGL181VEKKEMELVL SELEAAQRYL AQKYCILVLG LAVPDKHHMC CGKEKISDTQ KDWKFFESFY241TFCTYVAWIV FRRQHLTEIE EEVGRLFRTN MFNIPRRRRE DEESGGEKKR MTFVQFRRMM301AKRPAIKKAI NMRSPVMSTL LPSLREKAQN VFEKKYHQVD VRFPAEMQKH VGTLDSVPMP361VVGILGEPRC LFNPHTLHPL DPEENTKSFG RYPSLMENNN MRIQDTLDLV MKTLSSHTSC421PKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
422 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.