Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PPP1R21 / KLRAQ1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105963-100
Catalog Number: LS-G105963
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLRAQ(PPP1R21) (NM_152994), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCDC128 | KLRAQ1 | KLRAQ motif containing 1 | PPP1R21
- Origin Species
- Human
- NCBI GenBank
- R89358
- SwissProt
- Q6ZMI0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASAELQGKY QKLAQEYSKL RAQNQVLKKG VVDEQANSAA LKEQLKMKDQ SLRKLQQEMD61SLTFRNLQLA KRVELLQDEL ALSEPRGKKN KKSGESSSQL SQEQKSVFDE DLQKKIEENE121RLHIQFFEAD EQHKHVEAEL RSRLATLETE AAQHQAVVDG LTRKYMETIE KLQNDKAKLE181VKSQTLEKEA KECRLRTEEC QLQLKTLHED LSGRLEESLS IINEKVPFND TKYSQYNALN241VPLHNRRHQL KMRDIAGQAL AFVQDLVTAL LNFHTYTEQR IQIFPVDSAI DTISPLNQKF301SQYLHENASY VRPLEEGMLH LFESITEDTV TVLETTVKLK TFSEHLTSYI CFLRKILPYQ361LKSLEEECES SLCTSALRAR NLELSQDMKK MTAVFEKLQT YIALLALPST EPDGLLRTNY421SSVLTNVGAA LHGFHDVMKD ISKHYSQKAA IEHELPTATQ KLITTNDCIL SSVVALTNGA481GKIASFFSNN LDYFIASLSY GPKAASGFIS PLSAECMLQY KKKAAAYMKS LRKPLLESVP541YEEALANRRI LLSSTESREG LAQQVQQSLE KISKLEQEKE HWMLEAQLAK IKLEKENQRI601ADKLKNTGSA QLVGLAQENA AVSNTAGQDE ATAKAVLEPI QSTSLVPDVE SREDLIKNHY661MARIVELTSQ LQLADSKSVH FYAECRALSK RLALAEKSKE ALTEEMKLAS QNISRLQDEL721TTTKRSYEDQ LSMMSDHLCS MNETLSKQRE EIDTLKMSSK GNSKKNKSRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
769 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.