LSBio
PPP1R18 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP1R18 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPP1R18 antibodies →
$725.00each
Catalog Number: LS-G107513-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Phostensin(PPP1R18) (NM_133471), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HKMT1098 | Phostensin | PPP1R18 | KIAA1949
Origin Species
Human
NCBI GenBank
NM_001134870
SwissProt
Q6NYC8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATIPDWKLQ LLARRRQEEA SVRGREKAER ERLSQMPAWK RGLLERRRAK LGLSPGEPSP
61VLGTVEAGPP DPDESAVLLE AIGPVHQNRF IRQERQQQQQ QQQRSEELLA ERKPGPLEAR
121ERRPSPGEMR DQSPKGRESR EERLSPRETR ERRLGIGGAQ ELSLRPLEAR DWRQSPGEVG
181DRSSRLSEAW KWRLSPGETP ERSLRLAESR EQSPRRKEVE SRLSPGESAY QKLGLTEAHK
241WRPDSRESQE QSLVQLEATE WRLRSGEERQ DYSEECGRKE EWPVPGVAPK ETAELSETLT
301REAQGNSSAG VEAAEQRPVE DGERGMKPTE GWKWTLNSGK AREWTPRDIE AQTQKPEPPE
361SAEKLLESPG VEAGEGEAEK EEAGAQGRPL RALQNCCSVP SPLPPEDAGT GGLRQQEEEA
421VELQPPPPAP LSPPPPAPTA PQPPGDPLMS RLFYGVKAGP GVGAPRRSGH TFTVNPRRSV
481PPATPATPTS PATVDAAVPG AGKKRYPTAE EILVLGGYLR LSRSCLAKGS PERHHKQLKI
541SFSETALETT YQYPSESSVL EELGPEPEVP SAPNPPAAQP DDEEDEEELL LLQPELQGGL
601RTKALIVDES CRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

613 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.