LSBio
PPP1R16B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP1R16B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPP1R16B antibodies →
$504.00each
Catalog Number: LS-G129964-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP1R16B (NM_001172735), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD4 | HTIMAP | TIMAP | CAAX box protein TIMAP | KIAA0823 | PPP1R16B
Origin Species
Human
NCBI GenBank
AB020630
SwissProt
Q96T49

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MASHVDLLTE LQLLEKVPTL ERLRAAQKRR AQQLKKWAQY EQDLQHRKRK HERKRSTGGR
61RKKVSFEASV ALLEASLRND AEEVRYFLKN KVSPDLCNED GLTALHQCCI DNFEEIVKLL
121LSHGANVNAK DNELWTPLHA AATCGHINLV KILVQYGADL LAVNSDGNMP YDLCEDEPTL
181DVIETCMAYQ GITQEKINEM RVAPEQQMIA DIHCMIAAGQ DLDWIDAQGA TLMQMAELLV
241SHGASLSART SMDEMPIDLC EEEEFKVLLL ELKHKHDVIM KSQLRHKSSL SRRTSSAGSR
301GKVVRRASLS DRTNLYRKEY EGEAILWQRS AAEDQRTSTY NGDIRETRTD QENKDPNPRL
361EKPVLLSEFP TKIPRGELDM PVENGLRAPV SAYQYALANG DVWKVHEVPD YSMAYGNPGV
421ADATPPWSSY KEQSPQTLLE LKRQRAAAKL LSHPFLSTHL GSSMARTGES SSEGKAPLIG
481GRTSPYSSNG TSVYYTVTSG DPPLLKFKAP IEEMEEKVHG CCRIS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

525 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.