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PPP1R16A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP1R16A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108559-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP1R16A (NM_032902), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MYPT3 | PPP1R16A
Origin Species
Human
NCBI GenBank
NM_032902
SwissProt
Q96I34

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEHLELLAE MPMVGRMSTQ ERLKHAQKRR AQQVKMWAQA EKEAQGKKGP GERPRKEAAS
61QGLLKQVLFP PSVVLLEAAA RNDLEEVRQF LGSGVSPDLA NEDGLTALHQ CCIDDFREMV
121QQLLEAGANI NACDSECWTP LHAAATCGHL HLVELLIASG ANLLAVNTDG NMPYDLCDDE
181QTLDCLETAM ADRGITQDSI EAARAVPELR MLDDIRSRLQ AGADLHAPLD HGATLLHVAA
241ANGFSEAAAL LLEHRASLSA KDQDGWEPLH AAAYWGQVPL VELLVAHGAD LNAKSLMDET
301PLDVCGDEEV RAKLLELKHK HDALLRAQSR QRSLLRRRTS SAGSRGKVVR RVSLTQRTDL
361YRKQHAQEAI VWQQPPPTSP EPPEDNDDRQ TGAELRPPPP EEDNPEVVRP HNGRVGGSPV
421RHLYSKRLDR SVSYQLSPLD STTPHTLVHD KAHHTLADLK RQRAAAKLQR PPPEGPESPE
481TAEPGLPGDT VTPQPDCGFR AGGDPPLLKL TAPAVEAPVE RRPCCLLM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

528 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.