LSBio
PPP1R15B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPP1R15B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPP1R15B antibodies →
$504.00each
Catalog Number: LS-G108616-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPP1R15B (NM_032833), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PPP1R15B | CREP
Origin Species
Human
NCBI GenBank
NM_032833
SwissProt
Q5SWA1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPGTGGSRK RLGPRAGFRF WPPFFPRRSQ AGSSKFPTPL GPENSGNPTL LSSAQPETRV
61SYWTKLLSQL LAPLPGLLQK VLIWSQLFGG MFPTRWLDFA GVYSALRALK GREKPAAPTA
121QKSLSSLQLD SSDPSVTSPL DWLEEGIHWQ YSPPDLKLEL KAKGSALDPA AQAFLLEQQL
181WGVELLPSSL QSRLYSNREL GSSPSGPLNI QRIDNFSVVS YLLNPSYLDC FPRLEVSYQN
241SDGNSEVVGF QTLTPESSCL REDHCHPQPL SAELIPASWQ GCPPLSTEGL PEIHHLRMKR
301LEFLQQASKG QDLPTPDQDN GYHSLEEEHS LLRMDPKHCR DNPTQFVPAA GDIPGNTQES
361TEEKIELLTT EVPLALEEES PSEGCPSSEI PMEKEPGEGR ISVVDYSYLE GDLPISARPA
421CSNKLIDYIL GGASSDLETS SDPEGEDWDE EAEDDGFDSD SSLSDSDLEQ DPEGLHLWNS
481FCSVDPYNPQ NFTATIQTAA RIVPEEPSDS EKDLSGKSDL ENSSQSGSLP ETPEHSSGEE
541DDWESSADEA ESLKLWNSFC NSDDPYNPLN FKAPFQTSGE NEKGCRDSET PSESIVAISE
601CHTLLSCKVQ LLGSQESECP DSVQRDVLSG GRHTHVKRKK VTFLEEVTEY YISGDEDRKG
661PWEEFARDGC RFQKRIQETE DAIGYCLTFE HRERMFNRLQ GTCFKGLNVL KQC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

713 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.