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PPM1H Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPM1H Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPM1H antibodies →
$725.00each
Catalog Number: LS-G111077-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPM1H (NM_020700), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1157 | NERPP-2C | Protein phosphatase 1H | PPM1H | ARHCL1 | URCC2
Origin Species
Human
NCBI GenBank
AB032983
SwissProt
Q9ULR3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLTRVKSAVA NFMGGIMAGS SGSEHGGGSC GGSDLPLRFP YGRPEFLGLS QDEVECSADH
61IARPILILKE TRRLPWATGY AEVINAGKST HNEDQASCEV LTVKKKAGAV TSTPNRNSSK
121RRSSLPNGEG LQLKENSESE GVSCHYWSLF DGHAGSGAAV VASRLLQHHI TEQLQDIVDI
181LKNSAVLPPT CLGEEPENTP ANSRTLTRAA SLRGGVGAPG SPSTPPTRFF TEKKIPHECL
241VIGALESAFK EMDLQIERER SSYNISGGCT ALIVICLLGK LYVANAGDSR AIIIRNGEII
301PMSSEFTPET ERQRLQYLAF MQPHLLGNEF THLEFPRRVQ RKELGKKMLY RDFNMTGWAY
361KTIEDEDLKF PLIYGEGKKA RVMATIGVTR GLGDHDLKVH DSNIYIKPFL SSAPEVRIYD
421LSKYDHGSDD VLILATDGLW DVLSNEEVAE AITQFLPNCD PDDPHRYTLA AQDLVMRARG
481VLKDRGWRIS NDRLGSGDDI SVYVIPLIHG NKLS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

514 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.