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PPM1F Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPM1F Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101242-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPM1F (NM_014634), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CAMKP | CaMKPase | FEM-2 | PP2C phosphatase | Protein fem-2 homolog | Protein phosphatase 1F | POPX2 | CaM-kinase phosphatase | HFEM-2 | KIAA0015 | Partner of PIX 2 | PPM1F
Origin Species
Human
NCBI GenBank
NM_014634
SwissProt
P49593

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSGAPQKSS PMASGAEETP GFLDTLLQDF PALLNPEDPL PWKAPGTVLS QEEVEGELAE
61LAMGFLGSRK APPPLAAALA HEAVSQLLQT DLSEFRKLPR EEEEEEEDDD EEKAPVTLLD
121AQSLAQSFFN RLWEVAGQWQ KQVPLAARAS QRQWLVSIHA IRNTRRKMED RHVSLPSFNQ
181LFGLSDPVNR AYFAVFDGHG GVDAARYAAV HVHTNAARQP ELPTDPEGAL REAFRRTDQM
241FLRKAKRERL QSGTTGVCAL IAGATLHVAW LGDSQVILVQ QGQVVKLMEP HRPERQDEKA
301RIEALGGFVS HMDCWRVNGT LAVSRAIGDV FQKPYVSGEA DAASRALTGS EDYLLLACDG
361FFDVVPHQEV VGLVQSHLTR QQGSGLRVAE ELVAAARERG SHDNITVMVV FLRDPQELLE
421GGNQGEGDPQ AEGRRQDLPS SLPEPETQAP PRS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

453 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.