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PPFIBP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPFIBP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G100081-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPFIBP2 (NM_003621), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cclp1 | Liprin-beta-2 | PPFIBP2
Origin Species
Human
NCBI GenBank
AF034803
SwissProt
Q8ND30

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
98.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASDASHALE AALEQMDGII AGTKTGADLS DGTCEPGLAS PASYMNPFPV LHLIEDLRLA
61LEMLELPQER AALLSQIPGP TAAYIKEWFE ESLSQVNHHS AASNETYQER LARLEGDKES
121LILQVSVITD QVEAQGEKIR DLEVCLEGHQ VKLNAAEEML QQELLSRTSL ETQKLDLMTE
181VSELKLKLVG MEKEQREQEE KQRKAEELLQ ELRHLKIKVE ELENERNQYE WKLKATKAEV
241AQLQEQVALK DAEIERLHSQ LSRTAALHSE SHTERDQEIQ RLKMGMETLL LANEDKDRRI
301EELTGLLNQY RKVKEIVMVT QGPSERTLSI NEEEPEGGFS KWNATNKDPE ELFKQEMPPR
361CSSPTVGPPP LPQKSLETRA QKKLSCSLED LRSESVDKCM DGNQPFPVLE PKDSPFLAEH
421KYPTLPGKLS GATPNGEAAK SPPTICQPDA TGSSLLRLRD TESGWDDTAV VNDLSSTSSG
481TESGPQSPLT PDGKRNPKGI KKFWGKIRRT QSGNFYTDTL GMAEFRRGGL RATAGPRLSR
541TRDSKGQKSD ANAPFAQWST ERVCAWLEDF GLAQYVIFAR QWVSSGHTLL TATPQDMEKE
601LGIKHPLHRK KLVLAVKAIN TKQEEKSALL DHIWVTRWLD DIGLPQYKDQ FHESRVDGRM
661LQYLTVNDLL FLKVTSQLHH LSIKCAIHVL HVNKFNPHCL HRRPADESNL SPSEVVQWSN
721HRVMEWLRSV DLAEYAPNLR GSGVHGGLII LEPRFTGDTL AMLLNIPPQK TLLRRHLTTK
781FNALIGPEAE QEKREKMASP AYTPLTTTAK VRPRKLGFSH FGNIRKKKFD ESTDYICPME
841PSDGVSDSHR VYSGYRGLSP LDAPELDGLD QVGQIS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

876 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.