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PPAN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PPAN Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PPAN antibodies →
$725.00each
Catalog Number: LS-G120371-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PPAN (NM_001040664), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BXDC3 | Peter Pan homolog | PPAN | SSF | SSF2 | Peter pan (Drosophila) homolog | Second-step splicing factor 1 | Suppressor of sterile four 1 | Suppressor of SWI4 1 homolog | Homolog of S. cerevisiae SSF1 | Peter pan homolog (Drosophila) | SSF-1 | SSF1
Origin Species
Human
NCBI GenBank
NM_020230
SwissProt
Q9NQ55

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGQSGRSRHQ KRARAQAQLR NLEAYAANPH SFVFTRGCTG RNIRQLSLDV RRVMEPLTAS
61RLQVRKKNSL KDCVAVAGPL GVTHFLILSK TETNVYFKLM RLPGGPTLTF QVKKYSLVRD
121VVSSLRRHRM HEQQFAHPPL LVLNSFGPHG MHVKLMATMF QNLFPSINVH KVNLNTIKRC
181LLIDYNPDSQ ELDFRHYSIK VVPVGASRGM KKLLQEKFPN MSRLQDISEL LATGAGLSES
241EAEPDGDHNI TELPQAVAGR GNMRAQQSAV RLTEIGPRMT LQLIKVQEGV GEGKVMFHSF
301VSKTEEELQA ILEAKEKKLR LKAQRQAQQA QNVQRKQEQR EAHRKKSLEG MKKARVGGSD
361EEASGIPSRT ASLELGEDDD EQEDDDIEYF CQAVGEAPSE DLFPEAKQKR LAKSPGRKRK
421RWEMDRGAKS CPANFLAAAD DKLSGFQGDF LWPILVVEFL VAVASNGLAL YRFSIRKQRP
481WHPAVVFSVQ LAVSDLLCAL TLPPLAAYLY PPKHWRYGEA ACRLERFLFT CNLLGSVIFI
541TCISLNRYLG IVHPFFARSH LRPKHAWAVS AAGWVLAALL AMPTLSFSHL KRPQQGAGNC
601SVARPEACIK CLGTADHGLA AYRAYSLVLA GLGCGLPLLL TLAAYGALGR AVLRSPGMTV
661AEKLRVAALV ASGVALYASS YVPYHIMRVL NVDARRRWST RCPSFADIAQ ATAALELGPY
721VGYQVMRGLM PLAFCVHPLL YMAAVPSLGC CCRHCPGYRD SWNPEDAKST GQALPLNATA
781APKPSEPQSR ELSQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

794 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.