Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
POU4F1 / BRN3A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115444-100
Catalog Number: LS-G115444
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BRN3A(POU4F1) (NM_006237), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brain-3A | BRN3A | Brn-3A | Oct-T1 | POU class 4 homeobox 1 | POU4F1 | RDC1 | RDC-1
- Origin Species
- Human
- NCBI GenBank
- NM_006237
- SwissProt
- Q01851
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MMSMNSKQPH FAMHPTLPEH KYPSLHSSSE AIRRACLPTP PLQSNLFASL DETLLARAEA61LAAVDIAVSQ GKSHPFKPDA TYHTMNSVPC TSTSTVPLAH HHHHHHHHQA LEPGDLLDHI121SSPSLALMAG AGGAGAAAGG GGAHDGPGGG GGPGGGGGPG GGPGGGGGGG PGGGGGGPGG181GLLGGSAHPH PHMHSLGHLS HPAAAAAMNM PSGLPHPGLV AAAAHHGAAA AAAAAAAGQV241AAASAAAAVV GAAGLASICD SDTDPRELEA FAERFKQRRI KLGVTQADVG SALANLKIPG301VGSLSQSTIC RFESLTLSHN NMIALKPILQ AWLEEAEGAQ REKMNKPELF NGGEKKRKRT361SIAAPEKRSL EAYFAVQPRP SSEKIAAIAE KLDLKKNVVR VWFCNQRQKQ KRMKFSATYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
419 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.