Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
POU3F1 / OCT6 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117819-100
Catalog Number: LS-G117819
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Oct6(POU3F1) (NM_002699), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Oct-6 | OTF-6 | OTF6 | POU class 3 homeobox 1 | SCIP | OCT6 | Octamer-binding protein 6 | POU3F1
- Origin Species
- Human
- NCBI GenBank
- NM_002699
- SwissProt
- Q03052
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATTAQYLPR GPGGGAGGTG PLMHPDAAAA AAAAAAAERL HAGAAYREVQ KLMHHEWLGA61GAGHPVGLAH PQWLPTGGGG GGDWAGGPHL EHGKAGGGGT GRADDGGGGG GFHARLVHQG121AAHAGAAWAQ GSTAHHLGPA MSPSPGASGG HQPQPLGLYA QAAYPGGGGG GLAGMLAAGG181GGAGPGLHHA LHEDGHEAQL EPSPPPHLGA HGHAHGHAHA GGLHAAAAHL HPGAGGGGSS241VGEHSDEDAP SSDDLEQFAK QFKQRRIKLG FTQADVGLAL GTLYGNVFSQ TTICRFEALQ301LSFKNMCKLK PLLNKWLEET DSSSGSPTNL DKIAAQGRKR KKRTSIEVGV KGALESHFLK361CPKPSAHEIT GLADSLQLEK EVVRVWFCNR RQKEKRMTPA AGAGHPPMDD VYAPGELGPG421GGGASPPSAP PPPPPAALHH HHHHTLPGSV QTRTRPLEQKLISEEDLAANDILDYKDDDDKV
451 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.