Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
POU2F3 / PLA-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114001-100
Catalog Number: LS-G114001
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POU2F3 (NM_014352), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Epoc-1 | Octamer-binding protein 11 | POU class 2 homeobox 3 | Transcription factor PLA-1 | Transcription factor Skn-1 | PLA-1 | PLA1 | POU2F3 | OCT-11 | OCT11 | OTF-11 | OTF11 | Skn-1a
- Origin Species
- Human
- NCBI GenBank
- NM_014352
- SwissProt
- Q9UKI9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVNLESMHTD IKMSGDVADS TDARSTLSQV EPGNDRNGLD FNRQIKTEDL SDSLQQTLSH61RPCHLSQGPA MMSGNQMSGL NASPCQDMAS LHPLQQLVLV PGHLQSVSQF LLSQTQPGQQ121GLQPNLLPFP QQQSGLLLPQ TGPGLASQAF GHPGLPGSSL EPHLEASQHL PVPKHLPSSG181GADEPSDLEE LEKFAKTFKQ RRIKLGFTQG DVGLAMGKLY GNDFSQTTIS RFEALNLSFK241NMCKLKPLLE KWLNDAESSP SDPSVSTPSS YPSLSEVFGR KRKKRTSIET NIRLTLEKRF301QDNPKPSSEE ISMIAEQLSM EKEVVRVWFC NRRQKEKRIN CPVATPIKPP VYNSRLVSPS361GSLGPLSVPP VHSTMPGTVT SSCSPGNNSR PSSPGSGLHA SSPTASQNNS KAAVNSASSF421NSSGSWYRWN HSTYLHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
436 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.