Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
POU2F1 / OCT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117817-100
Catalog Number: LS-G117817
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Oct-1(POU2F1) (NM_002697), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NF-A1 | OCT1 | Octamer-binding protein 1 | Oct-1B | Oct-1 | POU2F1 | OTF-1 | OTF1 | POU class 2 homeobox 1
- Origin Species
- Human
- NCBI GenBank
- NM_002697
- SwissProt
- P14859
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNNPSETSKP SMESGDGNTG TQTNGLDFQK QPVPVGGAIS TAQAQAFLGH LHQVQLAGTS61LQAAAQSLNV QSKSNEESGD SQQPSQPSQQ PSVQAAIPQT QLMLAGGQIT GLTLTPAQQQ121LLLQQAQAQA QLLAAAVQQH SASQQHSAAG ATISASAATP MTQIPLSQPI QIAQDLQQLQ181QLQQQNLNLQ QFVLVHPTTN LQPAQFIISQ TPQGQQGLLQ AQNLLTQLPQ QSQANLLQSQ241PSITLTSQPA TPTRTIAATP IQTLPQSQST PKRIDTPSLE EPSDLEELEQ FAKTFKQRRI301KLGFTQGDVG LAMGKLYGND FSQTTISRFE ALNLSFKNMC KLKPLLEKWL NDAENLSSDS361SLSSPSALNS PGIEGLSRRR KKRTSIETNI RVALEKSFLE NQKPTSEEIT MIADQLNMEK421EVIRVWFCNR RQKEKRINPP SSGGTSSSPI KAIFPSPTSL VATTPSLVTS SAATTLTVSP481VLPLTSAAVT NLSVTGTSDT TSNNTATVIS TAPPASSAVT SPSLSPSPSA SASTSEASSA541SETSTTQTTS TPLSSPLGTS QVMVTASGLQ TAAAAALQGA AQLPANASLA AMAAAAGLNP601SLMAPSQFAA GGALLSLNPG TLSGALSPAL MSNSTLATIQ ALASGGSLPI TSLDATGNLV661FANAGGAPNI VTAPLFLNPQ NLSLLTSNPV SLVSAAAASA GNSAPVASLH ATSTSAESIQ721NSLFTVASAS GAASTTTTAS KAQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
743 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.