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POTEE Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

POTEE Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119807-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POTEE (NM_001083538), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
A26C1 | A26C1A | CT104.2 | POTE2gamma | POTE-2 | POTE2 | POTEE
Origin Species
Human
SwissProt
Q6S8J3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
121.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVVEVDSMPA ASSVKKPFGL RSKMGKWCCR CFPCYRESGK SNVGTSGDHD DSAMKTLRSK
61MGKWCHHCFP CCRGSGKSNV GASGDHDDSA MKTLRNKMGK WCCHCFPCCR GSGKSKVGAW
121GDYDDSAFME PRYHVRGEDL DKLHRAAWWG KVPRKDLIVM LRDTDVNKKD KQKRTALHLA
181SANGNSEVVK LLLDRRCQLN VLDNKKRTAL IKAVQCQEDE CALMLLEHGT DPNIPDEYGN
241TTLHYAIYNE DKLMAKALLL YGADIESKNK HGLTPLLLGV HEQKQQVVKF LIKKKANLNA
301LDRYGRTALI LAVCCGSASI VSLLLEQNID VSSQDLSGQT AREYAVSSHH HVICQLLSDY
361KEKQMLKISS ENSNPEQELK LTSEEESQRF KGSENSQPEK MSQELEINKD GDREVEEEMK
421KHESNNVGLL ENLTNGVTAG NGDNGLIPQR KSRTPENQQF PDNESEEYHR ICELLSDYKE
481KQMPKYSSEN SNPEQDLKLT SEEESQRLKG SENGQPEKRS QEPEINKDGD RELENFMAIE
541EMKKHGSTHV GFPENLTNGA TAGNGDDGLI PPRKSRTPES QQFPDTENEE YHSDEQNDTQ
601KQFCEEQNTG ILHDEILIHE EKQIEVVEKM NSELSLSCKK EKDVLHENST LREEIAMLRL
661ELDTMKHQSQ LREKKYLEDI ESVKKKNDNL LKALQLNELT MDDDTAVLVI DNGSGMCKAG
721FAGDDAPRAV FPSIVGRPRQ QGMMGGMHQK ESYVGKEAQS KRGILTLKYP MEHGIITNWD
781DMEKIWHHTF YNELRVAPEE HPILLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVP
841SLYTSGRTTG IVMDSGDGVT HTVPIYEGNA LPHATLRLDL AGRELPDYLM KILTERGYRF
901TTMAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA
961LFQPCFLGME SCGIHETTFN SIMKSDVDIR KDLYTNTVLS GGTTMYPGMA HRMQKEIAAL
1021APSMMKIRII APPKRKYSVW VGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1075 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.