Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
POTED / POTE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105110-100
Catalog Number: LS-G105110
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POTED (NM_174981), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A26B3 | Ankyrin repeat domain 21 | ANKRD21 | POTE | Protein POTE | ANKRD26-like family B member 3 | POTE-21 | POTED | CT104.1 | POTE21
- Origin Species
- Human
- NCBI GenBank
- NM_174981
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MVAEVCSMPT ASTVKKPFDL RSKMGKWCHH RFPCCRGSGK SNMGTSGDHD DSFMKMLRSK61MGKCCRHCFP CCRGSGTSNV GTSGDHENSF MKMLRSKMGK WCCHCFPCCR GSSKSNVGAW121GDYDHSAFME PRYHVRREDL DKLHRAAWWG KVPRKDLIVM LRDTDMNKRD KEKRTALHLA181SANGNSEVVQ LLLDRRCQLN VLDNKKRTAL IKAIQCQEDE CVLMLLEHGA DRNIPDEYGN241TALHYAIYNE DKLMAKALLL YGADIESKNK CGLTPLLLGV HEQKQQVVKF LIKKKANLNV301LDRYGRTALI LAVCCGSASI VNLLLEQNVD VSSQDLSGQT AREYAVSSHH HVICELLSDY361KEKQMLKISS ENSNPEQDLK LTSEEESQRL KVSENSQPEK MSQEPEINKD CDREVEEEIK421KHGSNPVGLP ENLTNGASAG NGDDGLIPQR RSRKPENQQF PDTENEEYHS DEQNDTRKQL481SEEQNTGISQ DEILTNKQKQ IEVAEQKMNS ELSLSHKKEE DLLRENSVLQ EEIAVLRLEL541DETKHQNQLR ENKILEEIES VKEKTDKLLR AMQLNEEALT KTNITRTRPLEQKLISEEDLAANDILDYKDDDDKV
584 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.