LSBio
POTEC Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

POTEC Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all POTEC antibodies →
$504.00each
Catalog Number: LS-G125792-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POTEC (NM_001137671), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001137671

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVTEVCSMPA ASAVKKPFDL RSKMGKWFHH RFPCCKGSGK SNMGTSGDHD DSFMKMLRSK
61MGKCCHHCFP CCRGSGTSNV GTSGDHDNSF MKTLRSKMGK WCCHCFPCCR GSGKSNVGAW
121GDYDDSAFME PRYHVRREDL DKLHRAAWWG KVPRKDLIVM LRDTDMNKRD KQKRTALHLA
181SANGNSEVVQ LLLDRRCQLN VLDNKKRTAL IKAVQCQEDE CVLMLLEHGA DQNIPDEYGN
241TTLHYAVHNE DKLMAKALLL YGADIESKNK CGLTPLLLGV HEQKQQVVKF LIKKKANLNA
301LDRYGRTALI LAVCCGSASI VNLLLEQNVD VSSQDLSGQT AREYAVSSHH HVICELLSDY
361KEKQMLKISS ENSNPEQDLK LTSEEESQRL KVSENSQPEK MSQEPEINKD CDREVEEEIK
421KHGSNPVGLP ENLTNGASAG NGDDGLIPQR RSRKPENQQF PDTENEEYHS DEQNDTRKQL
481SEEQNTGISQ DEILTNKQKQ IEVAEKKMNS ELSLSHKKEE DLLRENSMLQ EEIAMLISGD
541WN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

542 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.