Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
POTEB3 / POTE-15 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102769-100
Catalog Number: LS-G102769
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POTEB3 (NM_207355), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A26B1 | CT104.5 | POTE15 | ANKRD26-like family B member 1 | POTE-15 | POTEB
- Origin Species
- Human
- NCBI GenBank
- NM_207355
- SwissProt
- Q6S5H4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVAEVCSMPA ASAVKKPFDL RSKMGKWCHH RFPCCRGSGK SNMGTSGDHD DSFMKTLRSK61MGKCCHHCFP CCRGSGTSNV GTSGDHDNSF MKTLRSKMGK WCCHCFPCCR GSGKSNVGTW121GDYDDSAFME PRYHVRREDL DKLHRAAWWG KVPRKDLIVM LRDTDMNKRD KQKRTALHLA181SANGNSEVVQ LLLDRRCQLN VLDNKKRTAL IKAVQCQEDE CVLMLLEHGA DGNIQDEYGN241TALHYAIYNE DKLMAKALLL YGADIESKNK CGLTPLLLGV HEQKQQVVKF LIKKKANLNA301LDRYGRTALI LAVCCGSASI VNLLLEQNVD VSSQDLSGQT AREYAVSSHH HVICELLSDY361KEKQMLKISS ENSNPEQDLK LTSEEESQRL KVSENSQPEK MSQEPEINKD CDREVEEEIK421KHGSNPVGLP ENLTNGASAG NGDDGLIPQR KSRKPENQQF PDTENEEYHS DEQNDTQKQL481SEEQNTGISQ DEILTNKQKQ IEVAEKEMNS ELSLSHKKEE DLLRENSMLR EEIAKLRLEL541DETKHQNQLR ENKILEEIES VKEKLLKTIQ LNEEALTKTS ITRTRPLEQKLISEEDLAANDILDYKDDDDKV
581 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.