Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
POTEA / POTE8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122197-100
Catalog Number: LS-G122197
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POTEA (NM_001005365), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A26A1 | ANKRD26-like family A member 1 | CT104.3 | POTE-8 | POTE8 | POTEA
- Origin Species
- Human
- SwissProt
- Q6S8J7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVAEVSPKLA ASPMKKPFGF RGKMGKWCCC CFPCCRGSGK NNMGAWRDHD DSAFTEPRYH61VRREDLGKLH RAAWWGEVPR ADLIVMLRGP GINKRDKKKR TALHLACANG NSEVVSLLLD121RQCQLHVFDS KKRTALIKAV QCQEDECALM LLQHGTDPNL PDMYGNTALH YAVYNEDKLM181AKTLLLYGAD IESKNKGGLT PLLLAVHGQK QRMVKFLIKK KANLNALDRF GRTALILAVR241CGSASIVSLL LQQNIDVFSQ DVFGQTAEDY AVSSHHSIIC QLLSDYKENQ MPNNSSGNSN301PEQDLKLTSE EEPQRLKGSE NSQHEKVTQE PDINKDCDRE VEEEMQKHGS NNVGLSENLT361DGAAAGNGDG GLVPQRKSRK HENQQFPNTE IEEYHRPEKK SNEKNKVKSQ IHSVDNLDDI421TWPSEIASED YDLLFSNYET FTLLIEQLKM DFNDSASLSK IQDAVISEEH LLELKNSHYE481QLTVEVEQME NMVHVLQKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
498 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.