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POMT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

POMT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G119976-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POMT1 (NM_001077366), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MDDGA1 | MDDGC1 | RT | MDDGB1 | POMT1 | LGMD2K
Origin Species
Human
NCBI GenBank
NM_007171
SwissProt
Q9Y6A1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.57 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWGFLKRPVV VTADINLSLV ALTGMGLLSR LWRLTYPRAV VFDEVYYGQY ISFYMKQIFF
61LDDSGPPFGH MVLALGGYLG GFDGNFLWNR IGAEYSSNVP VWSLRLLPAL AGALSVPMAY
121QIVLELHFSH CAAMGAALLM LIENALITQS RLMLLESVLI FFNLLAVLSY LKFFNCQKHS
181PFSLSWWFWL TLTGVACSCA VGIKYMGVFT YVLVLGVAAV HAWHLLGDQT LSNVCVFCHL
241LARAVALLVI PVVLYLLFFY VHLILVFRSG PHDQIMSSAF QASLEGGLAR ITQGQPLEVA
301FGSQVTLRNV FGKPVPCWLH SHQDTYPMIY ENGRGSSHQQ QVTCYPFKDV NNWWIVKDPR
361RHQLVVSSPP RPVRHGDMVQ LVHGMTTRSL NTHDVAAPLS PHSQEVSCYI DYNISMPAQN
421LWRLEIVNRG SDTDVWKTIL SEVRFVHVNT SAVLKLSGAH LPDWGYRQLE IVGEKLSRGY
481HGSTVWNVEE HRYGASQEQR ERERELHSPA QVDVSRNLSF MARFSELQWR MLALRSDDSE
541HKYSSSPLEW VTLDTNIAYW LHPRTSAQIH LLGNIVIWVS GSLALAIYAL LSLWYLLRRR
601RNVHDLPQDA WLRWVLAGAL CAGGWAVNYL PFFLMEKTLF LYHYLPALTF QILLLPVVLQ
661HISDHLCRSQ LQRSIFSALV VAWYSSACHV SNTLRPLTYG DKSLSPHELK ALRWKDSWDI
721LIRKH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

725 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.