Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
POLR3E / SIN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111980-100
Catalog Number: LS-G111980
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POLR3E (NM_018119), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- POLR3E | KIAA1452 | RNA polymerase III subunit C5 | RPC5 | SIN
- Origin Species
- Human
- NCBI GenBank
- NM_018119
- SwissProt
- Q9NVU0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MANEEDDPVV QEIDVYLAKS LAEKLYLFQY PVRPASMTYD DIPHLSAKIK PKQQKVELEM61AIDTLNPNYC RSKGEQIALN VDGACADETS TYSSKLMDKQ TFCSSQTTSN TSRYAAALYR121QGELHLTPLH GILQLRPSFS YLDKADAKHR EREAANEAGD SSQDEAEDDV KQITVRFSRP181ESEQARQRRV QSYEFLQKKH AEEPWVHLHY YGLRDSRSEH ERQYLLCPGS SGVENTELVK241SPSEYLMMLM PPSQEEEKDK PVAPSNVLSM AQLRTLPLAD QIKILMKNVK VMPFANLMSL301LGPSIDSVAV LRGIQKVAML VQGNWVVKSD ILYPKDSSSP HSGVPAEVLC RGRDFVMWKF361TQSRWVVRKE VATVTKLCAE DVKDFLEHMA VVRINKGWEF ILPYDGEFIK KHPDVVQRQH421MLWTGIQAKL EKVYNLVKET MPKKPDAQSG PAGLVCGDQR IQVAKTKAQQ NHALLERELQ481RRKEQLRVPA VPPGVRIKEE PVSEEGEEDE EQEAEEEPMD TSPSGLHSKL ANGLPLGRAA541GTDSFNGHPP QGCASTPVAR ELKAFVEATF QRQFVLTLSE LKRLFNLHLA SLPPGHTLFS601GISDRMLQDT VLAAGCKQIL VPFPPQTAAS PDEQKVFALW ESGDMSDQHR QVLLEIFSKN661YRVRRNMIQS RLTQECGEDL SKQEVDKVLK DCCVSYGGMW YLKGTVQSTR RLEQKLISEEDLAANDILDYKDDDDKV
711 aa + 26 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.