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POLI Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

POLI Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all POLI antibodies →
$725.00each
Catalog Number: LS-G114793-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNA Polymerase iota(POLI) (NM_007195), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNA polymerase iota | Eta2 | POLI | Polymerase (DNA directed) iota | RAD30 homolog B | RAD3OB | RAD30B
Origin Species
Human
NCBI GenBank
NM_007195
SwissProt
Q9UNA4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.32 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELADVGAAA SSQGVHDQVL PTPNASSRVI VHVDLDCFYA QVEMISNPEL KDKPLGVQQK
61YLVVTCNYEA RKLGVKKLMN VRDAKEKCPQ LVLVNGEDLT RYREMSYKVT ELLEEFSPVV
121ERLGFDENFV DLTEMVEKRL QQLQSDELSA VTVSGHVYNN QSINLLDVLH IRLLVGSQIA
181AEMREAMYNQ LGLTGCAGVA SNKLLAKLVS GVFKPNQQTV LLPESCQHLI HSLNHIKEIP
241GIGYKTAKCL EALGINSVRD LQTFSPKILE KELGISVAQR IQKLSFGEDN SPVILSGPPQ
301SFSEEDSFKK CSSEVEAKNK IEELLASLLN RVCQDGRKPH TVRLIIRRYS SEKHYGRESR
361QCPIPSHVIQ KLGTGNYDVM TPMVDILMKL FRNMVNVKMP FHLTLLSVCF CNLKALNTAK
421KGLIDYYLMP SLSTTSRSGK HSFKMKDTHM EDFPKDKETN RDFLPSGRIE STRTRESPLD
481TTNFSKEKDI NEFPLCSLPE GVDQEVFKQL PVDIQEEILS GKSREKFQGK GSVSCPLHAS
541RGVLSFFSKK QMQDIPINPR DHLSSSKQVS SVSPCEPGTS GFNSSSSSYM SSQKDYSYYL
601DNRLKDERIS QGPKEPQGFH FTNSNPAVSA FHSFPNLQSE QLFSRNHTTD SHKQTVATDS
661HEGLTENREP DSVDEKITFP SDIDPQVFYE LPEAVQKELL AEWKRAGSDF HIGHK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

715 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.