Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
POLDIP3 / p46 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108905-100
Catalog Number: LS-G108905
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SKAR(POLDIP3) (NM_032311), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- p46 | KIAA1649 | SKAR | RNA-binding protein P46 | PDIP46 | POLDIP3 | S6K1 Aly/REF-like target
- Origin Species
- Human
- NCBI GenBank
- NM_032311
- SwissProt
- Q9BY77
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADISLDELI RKRGAAAKGR LNARPGVGGV RSRVGIQQGL LSQSTRTATF QQRFDARQKI61GLSDARLKLG VKDAREKLLQ KDARFRIKGK VQDAREMLNS RKQQTTVPQK PRQVADAREK121ISLKRSSPAA FINPPIGTVT PALKLTKTIQ VPQQKAMAPL HPHPAGMRIN VVNNHQAKQN181LYDLDEDDDG IASVPTKQMK FAASGGFLHH MAGLSSSKLS MSKALPLTKV VQNDAYTAPA241LPSSIRTKAL TNMSRTLVNK EEPPKELPAA EPVLSPLEGT KMTVNNLHPR VTEEDIVELF301CVCGALKRAR LVHPGVAEVV FVKKDDAITA YKKYNNRCLD GQPMKCNLHM NGNVITSDQP361ILLRLSDSPS MKKESELPRR VNSASSSNPP AEVDPDTILK ALFKSSGASV TTQPTEFKIK421LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
421 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.