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POGK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

POGK Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112403-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POGK (NM_017542), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BASS2 | LST003 | POGK | KIAA1513 | KRAB box domain containing 2 | KRBOX2 | KIAA15131
Origin Species
Human
NCBI GenBank
NM_017542
SwissProt
Q9P215

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESTAYPLNL SLKEEEEEEE IQSRELEDGP ADMQKVRICS EGGWVPALFD EVAIYFSDEE
61WEVLTEQQKA LYREVMRMNY ETVLSLEFPF PKPDMITRLE GEEESQNSDE WQLQGGTSAE
121NEESDVKPPD WPNPMNATSQ FPQPQHFDSF GLRLPRDITE LPEWSEGYPF YMAMGFPGYD
181LSADDIAGKF QFSRGMRRSY DAGFKLMVVE YAESTNNCQA AKQFGVLEKN VRDWRKVKPQ
241LQNAHAMRRA FRGPKNGRFA LVDQRVAEYV RYMQAKGDPI TREAMQLKAL EIAQEMNIPE
301KGFKASLGWC RRMMRRYDLS LRHKVPVPQH LPEDLTEKLV TYQRSVLALR RAHDYEVAQM
361GNADETPICL EVPSRVTVDN QGEKPVLVKT PGREKLKITA MLGVLADGRK LPPYIILRGT
421YIPPGKFPSG MEIRCHRYGW MTEDLMQDWL EVVWRRRTGA VPKQRGMLIL NGFRGHATDS
481VKNSMESMNT DMVIIPGGLT SQLQVLDVVV YKPLNDSVRA QYSNWLLAGN LALSPTGNAK
541KPPLGLFLEW VMVAWNSISS ESIVQGFKKC HISSNLEEED DVLWEIESEL PGGGEPPKDC
601DTESMAESN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

609 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.