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POF1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

POF1B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101922-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POF1B (NM_024921), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
POF2B | POF | Premature ovarian failure | 1B | POF1B | Protein POF1B
Origin Species
Human
NCBI GenBank
NM_024921
SwissProt
Q8WVV4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSSYWSETS SSSCGTQQLP EVLQCQPQHY HCYHQSSQAQ QPPEKNVVYE RVRTYSGPMN
61KVVQALDPFN SREVLSPLKT TSSYQNLVWS DHSQELHSPT LKISTCAPST LHITQNTEQE
121LHSPTVKLTT YPQTTIRKYV VQNPEQEPLS QFLRGSHFFP GNNVIYEKTI RKVEKLNTDQ
181GCHPQAQCHH HIIQQPQVIH SAHWQQPDSS QQIQAITGNN PISTHIGNEL CHSGSSQICE
241QVIIQDDGPE KLDPRYFGEL LADLSRKNTD LYHCLLEHLQ RIGGSKQDFE STDESEDIES
301LIPKGLSEFT KQQIRYILQM RGMSDKSLRL VLSTFSNIRE ELGHLQNDLT SLENDKMRLE
361KDLSFKDTQL KEYEELLASV RANNHQQQQG LQDSSSKCQA LEENNLSLRH TLSDMEYRLK
421ELEYCKRNLE QENQNLRMQV SETCTGPMLQ AKMDEIGNHY TEMVKNLRME KDREICRLRS
481QLNQYHKDVS KREGSCSDFQ FKLHELTSLL EEKDSLIKRQ SEELSKLRQE IYSSHNQPST
541GGRTTITTKK YRTQYPILGL LYDDYEYIPP GSETQTIVIE KTEDKYTCP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

589 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.