Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PODXL2 / Endoglycan Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113024-100
Catalog Number: LS-G113024
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Endoglycan(PODXL2) (NM_015720), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EG | Endoglycan | PODLX2 | Podocalyxin-like 2 | PODXL2 | Podocalyxin-like protein 2
- Origin Species
- Human
- NCBI GenBank
- NM_015720
- SwissProt
- Q9NZ53
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRLLRAARL PPLLSPLLLL LVGGAFLGAC VAGSDEPGPE GLTSTSLLDL LLPTGLEPLD61SEEPSETMGL GAGLGAPGSG FPSEENEESR ILQPPQYFWE EEEELNDSSL DLGPTADYVF121PDLTEKAGSI EDTSQAQELP NLPSPLPKMN LVEPPWHMPP REEEEEEEEE EEREKEEVEK181QEEEEEEELL PVNGSQEEAK PQVRDFSLTS SSQTPGATKS RHEDSGDQAS SGVEVESSMG241PSLLLPSVTP TTVTPGDQDS TSQEAEATVL PAAGLGVEFE APQEASEEAT AGAAGLSGQH301EEVPALPSFP QTTAPSGAEH PDEDPLGSRT SASSPLAPGD MELTPSSATL GQEDLNQQLL361EGQAAEAQSR IPWDSTQVIC KDWSNLAGKN YIILNMTENI DCEVFRQHRG PQLLALVEEV421LPRHGSGHHG AWHISLSKPS EKEQHLLMTL VGEQGVVPTQ DVLSMLGDIR RSLEEIGIQN481YSTTSSCQAR ASQVRSDYGT LFVVLVVIGA ICIIIIALGL LYNCWQRRLP KLKHVSHGEE541LRFVENGCHD NPTLDVASDS QSEMQEKHPS LNGGGALNGP GSWGALMGGK RDPEDSDVFE601EDTHLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
605 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.