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POC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

POC5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106299-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human POC5 (NM_152408), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C5orf37 | Centrosomal protein POC5 | HPOC5 | POC5 | Protein of centriole 5 | Proteome of centriole 5
Origin Species
Human
SwissProt
Q8NA72

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKWEEYEELL HYAIVTPNIE PCASQSSHPK GELVPDVRIS TIHDILHSQG NNSEVRETAI
61EVGKGCDFHI SSHSKTDESS PVLSPRKPSH PVMDFFSSHL LADSSSPATN SSHTDAHEIL
121VSDFLVSDEN LQKMENVLDL WSSGLKTNII SELSKWRLNF IDWHRMEMRK EKEKHAAHLK
181QLCNQINELK ELQKTFEISI GRKDEVISSL SHAIGKQKEK IELMRTFFHW RIGHVRARQD
241VYEGKLADQY YQRTLLKKVW KVWRSVVQKQ WKDVVERACQ ARAEEVCIQI SNDYEAKVAM
301LSGALENAKA EIQRMQHEKE HFEDSMKKAF MRGVCALNLE AMTIFQNRND AGIDSTNNKK
361EEYGPGVQGK EHSAHLDPSA PPMPLPVTSP LLPSPPAAVG GASATAVPSA ASMTSTRAAS
421ASSVHVPVSA LGAGSAATAA SEEMYVPRVV TSAQQKAGRT ITARITGRCD FASKNRISSS
481LAIMGVSPPM SSVVVEKHHP VTVQTIPQAT AAKYPRTIHP ESSTSASRSL GTRSAHTQSL
541TSVHSIKVVD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

550 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.