Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
POC1A / SOFT Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113257-100
Catalog Number: LS-G113257
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR51A(POC1A) (NM_015426), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DKFZP434C245 | PIX2 | POC1A | SOFT | WD repeat domain 51A | WDR51A
- Origin Species
- Human
- NCBI GenBank
- NM_015426
- SwissProt
- Q8NBT0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPCAEDPS LERHFKGHRD AVTCVDFSIN TKQLASGSMD SCLMVWHMKP QSRAYRFTGH61KDAVTCVNFS PSGHLLASGS RDKTVRIWVP NVKGESTVFR AHTATVRSVH FCSDGQSFVT121ASDDKTVKVW ATHRQKFLFS LSQHINWVRC AKFSPDGRLI VSASDDKTVK LWDKSSRECV181HSYCEHGGFV TYVDFHPSGT CIAAAGMDNT VKVWDVRTHR LLQHYQLHSA AVNGLSFHPS241GNYLITASSD STLKILDLME GRLLYTLHGH QGPATTVAFS RTGEYFASGG SDEQVMVWKS301NFDIVDHGEV TKVPRPPATL ASSMGNLPEV DFPVPPGRGR SVESVQSQPQ EPVSVPQTLT361STLEHIVGQL DVLTQTVSIL EQRLTLTEDK LKQCLENQQL IMQRATPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
407 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.