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PNMA3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PNMA3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114332-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PNMA3 (NM_013364), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MA3 | MA5 | Paraneoplastic antigen Ma3 | PNMA3 | Paraneoplastic Ma antigen 3
Origin Species
Human
NCBI GenBank
NM_013364
SwissProt
Q9UL41

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MPLTLLQDWC RGEHLNTRRC MLILGIPEDC GEDEFEETLQ EACRHLGRYR VIGRMFRREE
61NAQAILLELA QDIDYALLPR EIPGKGGPWE VIVKPRNSDG EFLNRLNRFL EEERRTVSDM
121NRVLGSDTNC SAPRVTISPE FWTWAQTLGA AVQPLLEQML YRELRVFSGN TISIPGALAF
181DAWLEHTTEM LQMWQVPEGE KRRRLMECLR GPALQVVSGL RASNASITVE ECLAALQQVF
241GPVESHKIAQ VKLCKAYQEA GEKVSSFVLR LEPLLQRAVE NNVVSRRNVN QTRLKRVLSG
301ATLPDKLRDK LKLMKQRRKP PGFLALVKLL REEEEWEATL GPDRESLEGL EVAPRPPARI
361TGVGAVPLPA SGNSFDARPS QGYRRRRGRG QHRRGGVARA GSRGSRKRKR HTFCYSCGED
421GHIRVQCINP SNLLLVKQKK QAAVESGNGN WAWDKSHPKS KAK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

463 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.