Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PNMA2 / MA2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114760-100
Catalog Number: LS-G114760
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MA2(PNMA2) (NM_007257), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 40 kDa neuronal protein | Onconeuronal antigen Ma2 | Paraneoplastic antigen Ma2 | PNMA2 | RGAG2 | MM2 | KIAA0883 | MA2 | Paraneoplastic Ma antigen 2
- Origin Species
- Human
- NCBI GenBank
- AB020690
- SwissProt
- Q9UL42
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MALALLEDWC RIMSVDEQKS LMVTGIPADF EEAEIQEVLQ ETLKSLGRYR LLGKIFRKQE61NANAVLLELL EDTDVSAIPS EVQGKGGVWK VIFKTPNQDT EFLERLNLFL EKEGQTVSGM121FRALGQEGVS PATVPCISPE LLAHLLGQAM AHAPQPLLPM RYRKLRVFSG SAVPAPEEES181FEVWLEQATE IVKEWPVTEA EKKRWLAESL RGPALDLMHI VQADNPSISV EECLEAFKQV241FGSLESRRTA QVRYLKTYQE EGEKVSAYVL RLETLLRRAV EKRAIPRRIA DQVSLEQVMA301GATLNQMLWC RLRELKDQGP PPSFLELMKV IREEEEEEAS FENESIEEPE ERDGYGRWNH361EGDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
364 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.