Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PNMA1 / MA1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115584-100
Catalog Number: LS-G115584
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PNMA1 (NM_006029), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 37 kDa neuronal protein | MA1 | Paraneoplastic antigen Ma1 | Paraneoplastic Ma antigen 1 | PNMA1
- Origin Species
- Human
- NCBI GenBank
- NM_006029
- SwissProt
- Q8ND90
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAMTLLEDWC RGMDVNSQRA LLVWGIPVNC DEAEIEETLQ AAMPQVSYRM LGRMFWREEN61AKAALLELTG AVDYAAIPRE MPGKGGVWKV LFKPPTSDAE FLERLHLFLA REGWTVQDVA121RVLGFQNPTP TPGPEMPAEM LNYILDNVIQ PLVESIWYKR LTLFSGRDIP GPGEETFDPW181LEHTNEVLEE WQVSDVEKRR RLMESLRGPA ADVIRILKSN NPAITTAECL KALEQVFGSV241ESSRDAQIKF LNTYQNPGEK LSAYVIRLEP LLQKVVEKGA IDKDNVNQAR LEQVIAGANH301SGAIRRQLWL TGAGEGPAPN LFQLLVQIRE EEAKEEEEEA EATLLQLGLE GHFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
353 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.