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PNLDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PNLDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PNLDC1 antibodies →
$725.00each
Catalog Number: LS-G105185-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PNLDC1 (NM_173516), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ195P10.2 | PNLDC1
Origin Species
Human
NCBI GenBank
NM_173516
SwissProt
Q8NA58

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFCTRGLLFF AFLAGLDIEF TGLRSNLSGP QQISLFDLPS EWYLKTRQSV QQFTVCQIGL
61SVFSAIEGEA NKYIAHSCNF YLFPTTFGIL DSEFSFQASS VQFLNQYGFN YNKFLKNGIP
121YMNEEQEKKI RHDILTGNWR VRSSPDKDQI KVVIDEVTRW LELAKEGDWM TLPGITGFQA
181FEVQLVLRQA LPNIWTVLKD EGVVVKKVSK QHRWYLQNTS CDRESCWKEN ILLSARGFSV
241FFQMLVKAQK PLVGHNMMMD LLHLHEKFFR PLPESYDQFK QNIHSLFPVL IDTKSVTKDI
301WKEMNFPRVS NLSEVYEVLN SDLNPTKNSG PEIVHASRCE KYVETKCPHE AAYDAFLCGS
361VLLKVAHLLL QKIYHIDPVP ESSFPQYLDV LAPYVNQVNL IRAGVPKINF SGPDYPSIRP
421PILILSVKRW PGVSEQQVYH KFQNLCKFDV RRLTRSQFLL LTNKFKDARN ILKEYRDHPT
481LCISLYRYWR HSPNVNCLLQ VCGIVTAWAL LAFILGRSGT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

520 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.