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PNISR Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PNISR Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113179-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PNISR (NM_015491), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA98I9.2 | C6orf111 | HSPC306 | SFRS18 | SR rich protein | SR-related protein | SR-rich protein | SRrp130 | PNISR | RP11-98I9.2
Origin Species
Human
NCBI GenBank
NM_015491
SwissProt
Q8TF01

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWDQGGQPWQ QWPLNQQQWM QSFQHQQDPS QIDWAALAQA WIAQREASGQ QSMVEQPPGM
61MPNGQDMSTM ESGPNNHGNF QGDSNFNRMW QPEWGMHQQP PHPPPDQPWM PPTPGPMDIV
121PPSEDSNSQD SGEFAPDNRH IFNQNNHNFG GPPDNFAVGP VNQFDYQHGA AFGPPQGGFH
181PPYWQPGPPG PPAPPQNRRE RPSSFRDRQR SPIALPVKQE PPQIDAVKRR TLPAWIREGL
241EKMEREKQKK LEKERMEQQR SQLSKKEKKA TEDAEGGDGP RLPQRSKFDS DEEEEDTENV
301EAASSGKVTR SPSPVPQEEH SDPEMTEEEK EYQMMLLTKM LLTEILLDVT DEEIYYVAKD
361AHRKATKAPA KQLAQSSALA SLTGLGGLGG YGSGDSEDER SDRGSESSDT DDEELRHRIR
421QKQEAFWRKE KEQQLLHDKQ MEEEKQQTER VTKEMNEFIH KEQNSLSLLE AREADGDVVN
481EKKRTPNETT SVLEPKKEHK EKEKQGRSRS GSSSSGSSSS NSRTSSTSST VSSSSYSSSS
541GSSRTSSRSS SPKRKKRHSR SRSPTIKARR SRSRSYSRRI KIESNRARVK IRDRRRSNRN
601SIERERRRNR SPSRERRRSR SRSRDRRTNR ASRSRSRDRR KIDDQRGNLS GNSHKHKGEA
661KEQERKKERS RSIDKDRKKK DKEREREQDK RKEKQKREEK DFKFSSQDDR LKRKRESERT
721FSRSGSISVK IIRHDSRQDS KKSTTKDSKK HSGSDSSGRS SSESPGSSKE KKAKKPKHSR
781SRSVEKSQRS GKKASRKHKS KSRSR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

805 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.