Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PNCK / CaMK1b Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128975-100
Catalog Number: LS-G128975
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PNCK (NM_001039582), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BSTK3 | CaM kinase I beta | CaM kinase IB | CaM kinase Ibeta1 | CaM-KI beta | CaMK1b | CaMKI-beta | PNCK | CaM kinase Ibeta2 | CaMKIbeta2
- Origin Species
- Human
- NCBI GenBank
- NM_001039582
- SwissProt
- Q6P2M8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAAFGQVAG SACPRRGGEG RDWKAESLAD LWPKSSPGDS HRWCKGPGAG PAGPQLREAA61RASSGLGGGG RHPSRIPAIA LQDMLLLKKH TEDISSVYEI RERLGSGAFS EVVLAQERGS121AHLVALKCIP KKALRGKEAL VENEIAVLRR ISHPNIVALE DVHESPSHLY LAMELVTGGE181LFDRIMERGS YTEKDASHLV GQVLGAVSYL HSLGIVHRDL KPENLLYATP FEDSKIMVSD241FGLSKIQAGN MLGTACGTPG YVAPELLEQK PYGKAVDVWA LGVISYILLC GYPPFYDESD301PELFSQILRA SYEFDSPFWD DISESAKDFI RHLLERDPQK RFTCQQALRH LWISGDTAFD361RDILGSVSEQ IRKNFARTHW KRAFNATSFL RHIRKLGQIP EGEGASEQGM ARHSHSGLRA421GQPPKWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
426 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.