Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLXNC1 / Plexin C1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115748-100
Catalog Number: LS-G115748
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLXNC1 (NM_005761), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Plexin C1 | Plexin-C1 | VESPR | Plexin (semaphorin receptor) | CD232 | CD232 antigen | PLXN-C1 | PLXNC1
- Origin Species
- Human
- NCBI GenBank
- NM_005761
- SwissProt
- O60486
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 175.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEVSRRKAPP RPPRPAAPLP LLAYLLALAA PGRGADEPVW RSEQAIGAIA ASQEDGVFVA61SGSCLDQLDY SLEHSLSRLY RDQAGNCTEP VSLAPPARPR PGSSFSKLLL PYREGAAGLG121GLLLTGWTFD RGACEVRPLG NLSRNSLRNG TEVVSCHPQG STAGVVYRAG RNNRWYLAVA181ATYVLPEPET ASRCNPAASD HDTAIALKDT EGRSLATQEL GRLKLCEGAG SLHFVDAFLW241NGSIYFPYYP YNYTSGAATG WPSMARIAQS TEVLFQGQAS LDCGHGHPDG RRLLLSSSLV301EALDVWAGVF SAAAGEGQER RSPTTTALCL FRMSEIQARA KRVSWDFKTA ESHCKEGDQP361ERVQPIASST LIHSDLTSVY GTVVMNRTVL FLGTGDGQLL KVILGENLTS NCPEVIYEIK421EETPVFYKLV PDPVKNIYIY LTAGKEVRRI RVANCNKHKS CSECLTATDP HCGWCHSLQR481CTFQGDCVHS ENLENWLDIS SGAKKCPKIQ IIRSSKEKTT VTMVGSFSPR HSKCMVKNVD541SSRELCQNKS QPNRTCTCSI PTRATYKDVS VVNVMFSFGS WNLSDRFNFT NCSSLKECPA601CVETGCAWCK SARRCIHPFT ACDPSDYERN QEQCPVAVEK TSGGGRPKEN KGNRTNQALQ661VFYIKSIEPQ KVSTLGKSNV IVTGANFTRA SNITMILKGT STCDKDVIQV SHVLNDTHMK721FSLPSSRKEM KDVCIQFDGG NCSSVGSLSY IALPHCSLIF PATTWISGGQ NITMMGRNFD781VIDNLIISHE LKGNINVSEY CVATYCGFLA PSLKSSKVRT NVTVKLRVQD TYLDCGTLQY841REDPRFTGYR VESEVDTELE VKIQKENDNF NISKKDIEIT LFHGENGQLN CSFENITRNQ901DLTTILCKIK GIKTASTIAN SSKKVRVKLG NLELYVEQES VPSTWYFLIV LPVLLVIVIF961AAVGVTRHKS KELSRKQSQQ LELLESELRK EIRDGFAELQ MDKLDVVDSF GTVPFLDYKH1021FALRTFFPES GGFTHIFTED MHNRDANDKN ESLTALDALI CNKSFLVTVI HTLEKQKNFS1081VKDRCLFASF LTIALQTKLV YLTSILEVLT RDLMEQCSNM QPKLMLRRTE SVVEKLLTNW1141MSVCLSGFLR ETVGEPFYLL VTTLNQKINK GPVDVITCKA LYTLNEDWLL WQVPEFSTVA1201LNVVFEKIPE NESADVCRNI SVNVLDCDTI GQAKEKIFQA FLSKNGSPYG LQLNEIGLEL1261QMGTRQKELL DIDSSSVILE DGITKLNTIG HYEISNGSTI KVFKKIANFT SDVEYSDDHC1321HLILPDSEAF QDVQGKRHRG KHKFKVKEMY LTKLLSTKVA IHSVLEKLFR SIWSLPNSRA1381PFAIKYFFDF LDAQAENKKI TDPDVVHIWK TNSLPLRFWV NILKNPQFVF DIKKTPHIDG1441CLSVIAQAFM DAFSLTEQQL GKEAPTNKLL YAKDIPTYKE EVKSYYKAIR DLPPLSSSEM1501EEFLTQESKK HENEFNEEVA LTEIYKYIVK YFDEILNKLE RERGLEEAQK QLLHVKVLFD1561EKKKCKWMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1568 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.