Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLXNB3 / Plexin B3 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128477-100
Catalog Number: LS-G128477
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLXNB3 (NM_001163257), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PLXNB3 | Plexin 6 | Plexin B3 | KIAA1206 | PLEXB3 | Plexin-B3 | PLEXR | PLXN6
- Origin Species
- Human
- NCBI GenBank
- NM_005393
- SwissProt
- Q9ULL4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 209.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MELTPASSLT CSLLSPRLPG SFPQLRRVPP CSRPWLPKAP VMARWPPFGL CLLLLLLSPP61PLPLTGAHRF SAPNTTLNHL ALAPGRGTLY VGAVNRLFQL SPELQLEAVA VTGPVIDSPD121CVPFRDPAEC PQAQLTDNAN QLLLVSSRAQ ELVACGQVRQ GVCETRRLGD VAEVLYQAED181PGDGQFVAAN TPGVATVGLV VPLPGRDLLL VARGLAGKLS AGVPPLAIRQ LAGSQPFSSE241GLGRLVVGDF SDYNNSYVGA FADARSAYFV FRRRGARAQA EYRSYVARVC LGDTNLYSYV301EVPLACQGQG LIQAAFLAPG TLLGVFAAGP RGTQAALCAF PMVELGASME QARRLCYTAG361GRGPSGAEEA TVEYGVTSRC VTLPLDSPES YPCGDEHTPS PIAGRQPLEV QPLLKLGQPV421SAVAALQADG HMIAFLGDTQ GQLYKVFLHG SQGQVYHSQQ VGPPGSAISP DLLLDSSGSH481LYVLTAHQVD RIPVAACPQF PDCASCLQAQ DPLCGWCVLQ GRCTRKGQCG RAGQLNQWLW541SYEEDSHCLH IQSLLPGHHP RQEQGQVTLS VPRLPILDAD EYFHCAFGDY DSLAHVEGPH601VACVTPPQDQ VPLNPPGTDH VTVPLALMFE DVTVAATNFS FYDCSAVQAL EAAAPCRACV661GSIWRCHWCP QSSHCVYGEH CPEGERTIYS AQEVDIQVRG PGACPQVEGL AGPHLVPVGW721ESHLALRVRN LQHFRGLPAS FHCWLELPGE LRGLPATLEE TAGDSGLIHC QAHQFYPSMS781QRELPVPIYV TQGEAQRLDN THALYVILYD CAMGHPDCSH CQAANRSLGC LWCADGQPAC841RYGPLCPPGA VELLCPAPSI DAVEPLTGPP EGGLALTILG SNLGRAFADV QYAVSVASRP901CNPEPSLYRT SARIVCVTSP APNGTTGPVR VAIKSQPPGI SSQHFTYQDP VLLSLSPRWG961PQAGGTQLTI RGQHLQTGGN TSAFVGGQPC PILEPVCPEA IVCRTRPQAA PGEAAVLVVF1021GHAQRTLLAS PFRYTANPQL VAAEPSASFR GGGRLIRVRG TGLDVVQRPL LSVWLEADAE1081VQASRAQPQD PQPRRSCGAP AADPQACIQL GGGLLQCSTV CSVNSSSLLL CRSPAVPDRA1141HPQRVFFTLD NVQVDFASAS GGQGFLYQPN PRLAPLSREG PARPYRLKPG HVLDVEGEGL1201NLGISKEEVR VHIGRGECLV KTLTRTHLYC EPPAHAPQPA NGSGLPQFVV QMGNVQLALG1261PVQYEAEPPL SAFPVEAQAG VGMGAAVLIA AVLLLTLMYR HKSKQALRDY QKVLVQLESL1321ETGVGDQCRK EFTDLMTEMT DLSSDLEGSG IPFLDYRTYA ERAFFPGHGG CPLQPKPEGP1381GEDGHCATVR QGLTQLSNLL NSKLFLLTLI HTLEEQPSFS QRDRCHVASL LSLALHGKLE1441YLTDIMRTLL GDLAAHYVHR NPKLMLRRTE TMVEKLLTNW LSICLYAFLR EVAGEPLYML1501FRAIQYQVDK GPVDAVTGKA KRTLNDSRLL REDVEFQPLT LMVLVGPGAG GAAGSSEMQR1561VPARVLDTDT ITQVKEKVLD QVYKGTPFSQ RPSVHALDLE WRSGLAGHLT LSDEDLTSVT1621QNHWKRLNTL QHYKVPDGAT VGLVPQLHRG STISQSLAQR CPLGENIPTL EDGEEGGVCL1681WHLVKATEEP EGAKVRCSSL REREPARAKA IPEIYLTRLL SMKGTLQKFV DDTFQAILSV1741NRPIPIAVKY LFDLLDELAE KHGIEDPGTL HIWKTNSLLL RFWVNALKNP QLIFDVRVSD1801NVDAILAVIA QTFIDSCTTS EHKVGRDSPV NKLLYAREIP RYKQMVERYY ADIRQSSPAS1861YQEMNSALAE LSGNYTSAPH CLEALQELYN HIHRYYDQII SALEEDPVGQ KLQLACRLQQ1921VAALVENKVT DLSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1932 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.