Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLXNA4 / Plexin A4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G127160-100
Catalog Number: LS-G127160
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Plexin A4(PLXNA4) (NM_020911), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1550 | PLXNA4A | Plexin A4 | A | Plexin-A4 | PLXNA4 | PLXNA4B | B | FAYV2820 | PLEXA4 | PRO34003
- Origin Species
- Human
- SwissProt
- Q9HCM2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 212.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKAMPWNWTC LLSHLLMVGM GSSTLLTRQP APLSQKQRSF VTFRGEPAEG FNHLVVDERT61GHIYLGAVNR IYKLSSDLKV LVTHETGPDE DNPKCYPPRI VQTCNEPLTT TNNVNKMLLI121DYKENRLIAC GSLYQGICKL LRLEDLFKLG EPYHKKEHYL SGVNESGSVF GVIVSYSNLD181DKLFIATAVD GKPEYFPTIS SRKLTKNSEA DGMFAYVFHD EFVASMIKIP SDTFTIIPDF241DIYYVYGFSS GNFVYFLTLQ PEMVSPPGST TKEQVYTSKL VRLCKEDTAF NSYVEVPIGC301ERSGVEYRLL QAAYLSKAGA VLGRTLGVHP DDDLLFTVFS KGQKRKMKSL DESALCIFIL361KQINDRIKER LQSCYRGEGT LDLAWLKVKD IPCSSALLTI DDNFCGLDMN APLGVSDMVR421GIPVFTEDRD RMTSVIAYVY KNHSLAFVGT KSGKLKKIRV DGPRGNALQY ETVQVVDPGP481VLRDMAFSKD HEQLYIMSER QLTRVPVESC GQYQSCGECL GSGDPHCGWC VLHNTCTRKE541RCERSKEPRR FASEMKQCVR LTVHPNNISV SQYNVLLVLE TYNVPELSAG VNCTFEDLSE601MDGLVVGNQI QCYSPAAKEV PRIITENGDH HVVQLQLKSK ETGMTFASTS FVFYNCSVHN661SCLSCVESPY RCHWCKYRHV CTHDPKTCSF QEGRVKLPED CPQLLRVDKI LVPVEVIKPI721TLKAKNLPQP QSGQRGYECI LNIQGSEQRV PALRFNSSSV QCQNTSYSYE GMEINNLPVE781LTVVWNGHFN IDNPAQNKVH LYKCGAMRES CGLCLKADPD FACGWCQGPG QCTLRQHCPA841QESQWLELSG AKSKCTNPRI TEIIPVTGPR EGGTKVTIRG ENLGLEFRDI ASHVKVAGVE901CSPLVDGYIP AEQIVCEMGE AKPSQHAGFV EICVAVCRPE FMARSSQLYY FMTLTLSDLK961PSRGPMSGGT QVTITGTNLN AGSNVVVMFG KQPCLFHRRS PSYIVCNTTS SDEVLEMKVS1021VQVDRAKIHQ DLVFQYVEDP TIVRIEPEWS IVSGNTPIAV WGTHLDLIQN PQIRAKHGGK1081EHINICEVLN ATEMTCQAPA LALGPDHQSD LTERPEEFGF ILDNVQSLLI LNKTNFTYYP1141NPVFEAFGPS GILELKPGTP IILKGKNLIP PVAGGNVKLN YTVLVGEKPC TVTVSDVQLL1201CESPNLIGRH KVMARVGGME YSPGMVYIAP DSPLSLPAIV SIAVAGGLLI IFIVAVLIAY1261KRKSRESDLT LKRLQMQMDN LESRVALECK EAFAELQTDI HELTSDLDGA GIPFLDYRTY1321TMRVLFPGIE DHPVLRDLEV PGYRQERVEK GLKLFAQLIN NKVFLLSFIR TLESQRSFSM1381RDRGNVASLI MTVLQSKLEY ATDVLKQLLA DLIDKNLESK NHPKLLLRRT ESVAEKMLTN1441WFTFLLYKFL KECAGEPLFS LFCAIKQQME KGPIDAITGE ARYSLSEDKL IRQQIDYKTL1501VLSCVSPDNA NSPEVPVKIL NCDTITQVKE KILDAIFKNV PCSHRPKAAD MDLEWRQGSG1561ARMILQDEDI TTKIENDWKR LNTLAHYQVP DGSVVALVSK QVTAYNAVNN STVSRTSASK1621YENMIRYTGS PDSLRSRTPM ITPDLESGVK MWHLVKNHEH GDQKEGDRGS KMVSEIYLTR1681LLATKGTLQK FVDDLFETIF STAHRGSALP LAIKYMFDFL DEQADKHGIH DPHVRHTWKS1741NCLPLRFWVN MIKNPQFVFD IHKNSITDAC LSVVAQTFMD SCSTSEHRLG KDSPSNKLLY1801AKDIPSYKNW VERYYSDIGK MPAISDQDMN AYLAEQSRMH MNEFNTMSAL SEIFSYVGKY1861SEEILGPLDH DDQCGKQKLA YKLEQVITLM SLDSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1894 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.