Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLXNA2 / Plexin A2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109548-100
Catalog Number: LS-G109548
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Plexin A2(PLXNA2) (NM_025179), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- OCT | Plexin 2 | KIAA0463 | Semaphorin receptor OCT | PLXN2 | Transmembrane protein OCT | Plexin A2 | Plexin-A2 | PLXNA2
- Origin Species
- Human
- NCBI GenBank
- D86949
- SwissProt
- P70207
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 210.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEQRRPWPRA LEVDSRSVVL LSVVWVLLAP PAAGMPQFST FHSENRDWTF NHLTVHQGTG61AVYVGAINRV YKLTGNLTIQ VAHKTGPEED NKSCYPPLIV QPCSEVLTLT NNVNKLLIID121YSENRLLACG SLYQGVCKLL RLDDLFILVE PSHKKEHYLS SVNKTGTMYG VIVRSEGEDG181KLFIGTAVDG KQDYFPTLSS RKLPRDPESS AMLDYELHSD FVSSLIKIPS DTLALVSHFD241IFYIYGFASG GFVYFLTVQP ETPEGVAINS AGDLFYTSRI VRLCKDDPKF HSYVSLPFGC301TRAGVEYRLL QAAYLAKPGD SLAQAFNITS QDDVLFAIFS KGQKQYHHPP DDSALCAFPI361RAINLQIKGR LQSCYQGEGN LELNWLLGKD VQCTKAPVPI DDNFCGLDIN QPLGGSTPVE421GLTLYTTSRD RMTSVASYVY NGYSVVFVGT KSGKLKKIRA DGPPHGGVQY EMVSVLKDGS481PILRDMAFSI DQRYLYVMSE RQVTRVPVES CEQYTTCGEC LSSGDPHCGW CALHNMCSRR541DKCQQAWEPN RFAASISQCV SLAVHPSSIS VSEHSRLLSL VVSDAPDLSA GIACAFGNLT601EVEGQVSGSQ VICISPGPKD VPVIPLDQDW FGLELQLRSK ETGKIFVSTE FKFYNCSAHQ661LCLSCVNSAF RCHWCKYRNL CTHDPTTCSF QEGRINISED CPQLVPTEEI LIPVGEVKPI721TLKARNLPQP QSGQRGYECV LNIQGAIHRV PALRFNSSSV QCQNSSYQYD GMDISNLAVD781FAVVWNGNFI IDNPQDLKVH LYKCAAQRES CGLCLKADRK FECGWCSGER RCTLHQHCTS841PSSPWLDWSS HNVKCSNPQI TEILTVSGPP EGGTRVTIHG VNLGLDFSEI AHHVQVAGVP901CTPLPGEYII AEQIVCEMGH ALVGTTSGPV RLCIGECKPE FMTKSHQQYT FVNPSVLSLN961PIRGPESGGT MVTITGHYLG AGSSVAVYLG NQTCEFYGRS MSEIVCVSPP SSNGLGPVPV1021SVSVDRAHVD SNLQFEYIDD PRVQRIEPEW SIASGHTPLT ITGFNLDVIQ EPRIRVKFNG1081KESVNVCKVV NTTTLTCLAP SLTTDYRPGL DTVERPDEFG FVFNNVQSLL IYNDTKFIYY1141PNPTFELLSP TGVLDQKPGS PIILKGKNLC PPASGGAKLN YTVLIGETPC AVTVSETQLL1201CEPPNLTGQH KVMVHVGGMV FSPGSVSVIS DSLLTLPAIV SIAAGGSLLL IIVIIVLIAY1261KRKSRENDLT LKRLQMQMDN LESRVALECK EAFAELQTDI NELTSDLDRS GIPYLDYRTY1321AMRVLFPGIE DHPVLRELEV QGNGQQHVEK ALKLFAQLIN NKVFLLTFIR TLELQRSFSM1381RDRGNVASLI MTGLQGRLEY ATDVLKQLLS DLIDKNLENK NHPKLLLRRT ESVAEKMLTN1441WFAFLLHKFL KECAGEPLFM LYCAIKQQME KGPIDAITGE ARYSLSEDKL IRQQIEYKTL1501ILNCVNPDNE NSPEIPVKVL NCDTITQVKE KILDAVYKNV PYSQRPRAVD MDLEWRQGRI1561ARVVLQDEDI TTKIEGDWKR LNTLMHYQVS DRSVVALVPK QTSSYNIPAS ASISRTSISR1621YDSSFRYTGS PDSLRSRAPM ITPDLESGVK VWHLVKNHDH GDQKEGDRGS KMVSEIYLTR1681LLATKGTLQK FVDDLFETLF STVHRGSALP LAIKYMFDFL DEQADRHSIH DTDVRHTWKS1741NCLPLRFWVN VIKNPQFVFD IHKGSITDAC LSVVAQTFMD SCSTSEHRLG KDSPSNKLLY1801AKDIPSYKSW VERYYADIAK LPAISDQDMN AYLAEQSRLH AVEFNMLSAL NEIYSYVSKY1861SEELIGALEQ DEQARRQRLA YKVEQLINAM SIESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1894 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.