Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLXNA1 / Plexin A1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108957-100
Catalog Number: LS-G108957
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Plexin A1(PLXNA1) (NM_032242), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NOVP | Plexin A1 | Plexin-A1 | PLXNA1 | Plexin 1 | PLXN1 | Semaphorin receptor NOV
- Origin Species
- Human
- NCBI GenBank
- NM_032242
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 211.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPLPPRSLQV LLLLLLLLLL LPGMWAEAGL PRAGGGSQPP FRTFSASDWG LTHLVVHEQT61GEVYVGAVNR IYKLSGNLTL LRAHVTGPVE DNEKCYPPPS VQSCPHGLGS TDNVNKLLLL121DYAANRLLAC GSASQGICQF LRLDDLFKLG EPHHRKEHYL SSVQEAGSMA GVLIAGPPGQ181GQAKLFVGTP IDGKSEYFPT LSSRRLMANE EDADMFGFVY QDEFVSSQLK IPSDTLSKFP241AFDIYYVYSF RSEQFVYYLT LQLDTQLTSP DAAGEHFFTS KIVRLCVDDP KFYSYVEFPI301GCEQAGVEYR LVQDAYLSRP GRALAHQLGL AEDEDVLFTV FAQGQKNRVK PPKESALCLF361TLRAIKEKIK ERIQSCYRGE GKLSLPWLLN KELGCINSPL QIDDDFCGQD FNQPLGGTVT421IEGTPLFVDK DDGLTAVAAY DYRGRTVVFA GTRSGRIRKI LVDLSNPGGR PALAYESVVA481QEGSPILRDL VLSPNHQYLY AMTEKQVTRV PVESCVQYTS CELCLGSRDP HCGWCVLHSI541CSRRDACERA DEPQRFAADL LQCVQLTVQP RNVSVTMSQV PLVLQAWNVP DLSAGVNCSF601EDFTESESVL EDGRIHCRSP SAREVAPITR GQGDQRVVKL YLKSKETGKK FASVDFVFYN661CSVHQSCLSC VNGSFPCHWC KYRHVCTHNV ADCAFLEGRV NVSEDCPQIL PSTQIYVPVG721VVKPITLAAR NLPQPQSGQR GYECLFHIPG SPARVTALRF NSSSLQCQNS SYSYEGNDVS781DLPVNLSVVW NGNFVIDNPQ NIQAHLYKCP ALRESCGLCL KADPRFECGW CVAERRCSLR841HHCAADTPAS WMHARHGSSR CTDPKILKLS PETGPRQGGT RLTITGENLG LRFEDVRLGV901RVGKVLCSPV ESEYISAEQI VCEIGDASSV RAHDALVEVC VRDCSPHYRA LSPKRFTFVT961PTFYRVSPSR GPLSGGTWIG IEGSHLNAGS DVAVSVGGRP CSFSWRNSRE IRCLTPPGQS1021PGSAPIIINI NRAQLTNPEV KYNYTEDPTI LRIDPEWSIN SGGTLLTVTG TNLATVREPR1081IRAKYGGIER ENGCLVYNDT TMVCRAPSVA NPVRSPPELG ERPDELGFVM DNVRSLLVLN1141STSFLYYPDP VLEPLSPTGL LELKPSSPLI LKGRNLLPPA PGNSRLNYTV LIGSTPCTLT1201VSETQLLCEA PNLTGQHKVT VRAGGFEFSP GTLQVYSDSL LTLPAIVGIG GGGGLLLLVI1261VAVLIAYKRK SRDADRTLKR LQLQMDNLES RVALECKEAF AELQTDIHEL TNDLDGAGIP1321FLDYRTYAMR VLFPGIEDHP VLKEMEVQAN VEKSLTLFGQ LLTKKHFLLT FIRTLEAQRS1381FSMRDRGNVA SLIMTALQGE MEYATGVLKQ LLSDLIEKNL ESKNHPKLLL RRTESVAEKM1441LTNWFTFLLY KFLKECAGEP LFMLYCAIKQ QMEKGPIDAI TGEARYSLSE DKLIRQQIDY1501KTLTLNCVNP ENENAPEVPV KGLDCDTVTQ AKEKLLDAAY KGVPYSQRPK AADMDLEWRQ1561GRMARIILQD EDVTTKIDND WKRLNTLAHY QVTDGSSVAL VPKQTSAYNI SNSSTFTKSL1621SRYESMLRTA SSPDSLRSRT PMITPDLESG TKLWHLVKNH DHLDQREGDR GSKMVSEIYL1681TRLLATKGTL QKFVDDLFET IFSTAHRGSA LPLAIKYMFD FLDEQADKHQ IHDADVRHTW1741KSNCLPLRFW VNVIKNPQFV FDIHKNSITD ACLSVVAQTF MDSCSTSEHK LGKDSPSNKL1801LYAKDIPNYK SWVERYYADI AKMPAISDQD MSAYLAEQSR LHLSQFNSMS ALHEIYSYIT1861KYKDEILAAL EKDEQARRQR LRSKLEQVVD TMALSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1896 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.