Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLIN5 / OXPAT / LSDP5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121542-100
Catalog Number: LS-G121542
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LSDP5(PLIN5) (NM_001013706), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LSDA5 | Perilipin 5 | Perilipin-5 | LSDP5 | OXPAT | MLDP | PLIN5
- Origin Species
- Human
- NCBI GenBank
- NM_001013706
- SwissProt
- Q00G26
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSEEEAAQIP RSSVWEQDQQ NVVQRVVALP LVRATCTAVC DVYSAAKDRH PLLGSACRLA61ENCVCGLTTR ALDHAQPLLE HLQPQLATMN SLACRGLDKL EEKLPFLQQP SETVVTSAKD121VVASSVTGVV DLARRGRRWS VELKRSVSHA VDVVLEKSEE LVDHFLPMTE EELAALAAEA181EGPEVGSVED QRRQQGYFVR LGSLSARIRH LAYEHSVGKL RQSKHRAQDT LAQLQETLEL241IDHMQCGVTP TAPACPGKVH ELWGEWGQRP PESRRRSQAE LETLVLSRSL TQELQGTVEA301LESSVRGLPA GAQEKVAEVR RSVDALQTAF ADARCFRDVP AAALAEGRGR VAHAHACVDE361LLELVVQAVP LPWLVGPFAP ILVERPEPLP DLADLVDEVI GGPDPRWAHL DWPAQQRAWE421AEHRDGSGNG DGDRMGVAGD ICEQEPETPS CPVKHTLMPE LDFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
463 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.