Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLIN3 / M6PRBP1 / TIP47 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128121-100
Catalog Number: LS-G128121
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Perilipin 3(PLIN3) (NM_001164194), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 47 kDa MPR-binding protein | Cargo selection protein TIP47 | Perilipin 3 | Placental protein 17 | PLIN3 | PP17 | M6PRBP1 | TIP47 | Perilipin-3
- Origin Species
- Human
- NCBI GenBank
- NM_005817
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSADGAEADG STQVTVEEPV QQPSVVDRVA SMPLISSTCD MVSAAYASTK ESYPHIKTVC61DAAEKGVRTL TAAAVSGAQP ILSKLEPQIA SASEYAHRGL DKLEENLPIL QQPTEKVSGA121QEMVSSAKDT VATQLSEAVD ATRGAVQSGV DKTKSVVTGG VQSVMGSRLG QMVLSGVDTV181LGKSEEWADN HLPLTDAELA RIATSLDGFD VASVQQQRQE QSYFVRLGSL SERLRQHAYE241HSLGKLRATK QRAQEALLQL SQVLSLMETV KQGVDQKLVE GQEKLHQMWL SWNQKQLQGP301EKEPPKPEQV ESRALTMFRD IAQQLQATCT SLGSSIQGLP TNVKDQVQQA RRQVEDLQAT361FSSIHSFQDL SSSILAQSRE RVASAREALD HMVEYVAQNT PVTWLVGPFA PGITEKAPEE421KKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
422 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.