Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLIN2 / ADFP / Adipophilin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99339-100
Catalog Number: LS-G99339
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADFP(PLIN2) (NM_001122), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Adipophilin | ADFP | ADRP | Perilipin 2 | PLIN2 | Perilipin-2 | Adipose differentiation-related protein
- NCBI GenBank
- NM_001122
- SwissProt
- Q99541
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASVAVDPQP SVVTRVVNLP LVSSTYDLMS SAYLSTKDQY PYLKSVCEMA ENGVKTITSV61AMTSALPIIQ KLEPQIAVAN TYACKGLDRI EERLPILNQP STQIVANAKG AVTGAKDAVT121TTVTGAKDSV ASTITGVMDK TKGAVTGSVE KTKSVVSGSI NTVLGSRMMQ LVSSGVENAL181TKSELLVEQY LPLTEEELEK EAKKVEGFDL VQKPSYYVRL GSLSTKLHSR AYQQALSRVK241EAKQKSQQTI SQLHSTVHLI EFARKNVYSA NQKIQDAQDK LYLSWVEWKR SIGYDDTDES301HCAEHIESRT LAIARNLTQQ LQTTCHTLLS NIQGVPQNIQ DQAKHMGVMA GDIYSVFRNA361ASFKEVSDSL LTSSKGQLQK MKESLDDVMD YLVNNTPLNW LVGPFYPQLT ESQNAQDQGA421EMDKSSQETQ RSEHKTHTRT RPLEQKLISE EDLAANDILD YKDDDDKV
468 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.