Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLEKHS1 / C10orf81 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130907-100
Catalog Number: LS-G130907
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C10orf81(PLEKHS1) (NM_001193434), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA211N11.2 | C10orf81 | Epididymis luminal protein 185 | RP11-211N11.2 | HEL185 | PLEKHS1
- Origin Species
- Human
- NCBI GenBank
- NM_024889
- SwissProt
- Q5SXH7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MQSVQKMFKC HPDEVMSIRT TNREYFLIGH DREKIKDWVS FMSSFRQDIK ATQQNTEEEL61SLGNKRTLFY SSPLLGPSST SEAVGSSSPR NGLQDKHLME QSSPGFRQTH LQDLSEATQD121VKEENHYLTP RSVLLELDNI IASSDSGESI ETDGPDQVSG RIECHYEPME SYFFKETSHE181SVDSSKEEPQ TLPETQDGDL HLQEQGSGID WCLSPADVEA QTTNDQKGNI PDESQVEKLN241VFLSPPDVIN YLALTEATGR ICVSQWEGPP RLGCIFCHGD HLLAVNDLKP QSLEEVSLFL301TRSIQKEKLK LTIGRIPNSE TFHAASCMCP SKCQSAAPSQ LDKPRLNRAP KRSPAIKKSQ361QKGARETRTRPLEQKLISEEDLAANDILDYKDDDDKV
366 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.