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PLEKHO2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLEKHO2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109540-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLEKHO2 (NM_025201), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PH domain-containing protein | PLEKHQ1 | PP1628 | PLEKHO2 | Pp9099
Origin Species
Human
NCBI GenBank
NM_025201
SwissProt
Q8TD55

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MEEEGVKEAG EKPRGAQMVD KAGWIKKSSG GLLGFWKDRY LLLCQAQLLV YENEDDQKCV
61ETVELGSYEK CQDLRALLKR KHRFILLRSP GNKVSDIKFQ APTGEEKESW IKALNEGINR
121GKNKAFDEVK VDKSCALEHV TRDRVRGGQR RRPPTRVHLK EVASAASDGL LRLDLDVPDS
181GPPVFAPSNH VSEAQPRETP RPLMPPTKPF LAPETTSPGD RVETPVGERA PTPVSASSEV
241SPESQEDSET PAEEDSGSEQ PPNSVLPDKL KVSWENPSPQ EAPAAESAEP SQAPCSETSE
301AAPREGGKPP TPPPKILSEK LKASMGEMQA SGPPAPGTVQ VSVNGMDDSP EPAKPSQAEG
361TPGTPPKDAT TSTALPPWDL PPQFHPRCSS LGDLLGEGPR HPLQPRERLY RAQLEVKVAS
421EQTEKLLNKV LGSEPAPVSA ETLLSQAVEQ LRQATQVLQE MRDLGELSQE APGLREKRKE
481LVTLYRRSAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

490 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.